TMRE (TetraMethylRhodamine Ethyl ester)

Structure and Spectra of TMRE

Structure of Di-4-ANEPPS, Potentiometric Probes
  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 560.5 nm, 588 nm.
  • Absorbance, emission peaks in ethanol: 551 nm, 578 nm.
  • MW: 515.0 g/mol

Unique Packaging for Convenience and Reliability

  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (52 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant
  • Bulk mg quantities also available
Packaging of Di-4-ANEPPS, Potentiometric Probes

Order information

  • See table for pricing
  • Contact for a quote
  • Minimum order: 20. Price break: 100 tubes
  • Shipping not included
  • Bulk 1 mg and 5mg packaging also available.
  • Inquire about “Academic Discount” for academic researchers
TMRE, 100 nmol Pre-dried Aliquot Pricing
Order Quantity (tubes) Price / Tube Order Total ($US)
20 $4.00 $80
100 $3.50 $350
TMRE, Bulk Pricing
Order Quantity (mg) Price / mg Order Total ($US)
1 $50 $50
5 $40 $200


  1. ​Ehrenberg, B., V. Montana, M. D. Wei, J. P. Wuskell, and L. M. Loew. 1988. Membrane potential can be determined in individual cells from the nernstian distribution of cationic dyes. Biophysical journal 53:785-794. PMC1330255    PubMed
  2. Farkas, D. L., M. D. Wei, P. Febbroriello, J. H. Carson, and L. M. Loew. 1989. Simultaneous imaging of cell and mitochondrial membrane potentials. Biophysical journal 56:1053-1069. PMC1280610   PubMed
  3. Loew, L. M., R. A. Tuft, W. Carrington, and F. S. Fay. 1993. Imaging in five dimensions: time-dependent membrane potentials in individual mitochondria. Biophysical journal 65:2396-2407. PMC1225980  PubMed
  4. Loew, L. M., W. Carrington, R. A. Tuft, and F. S. Fay. 1994. Physiological cytosolic Ca2+ transients evoke concurrent mitochondrial depolarizations. Proceedings of the National Academy of Sciences of the United States of America 91:12579-12583.45482
  5. Fink, C., F. Morgan, and L. M. Loew. 1998. Intracellular fluorescent probe concentrations by confocal microscopy. Biophysical journal 75:1648-1658. PMC1299837  PubMed
  6. Ward, M. W., A. C. Rego, B. G. Frenguelli, and D. G. Nicholls. 2000. Mitochondrial membrane potential and glutamate excitotoxicity in cultured cerebellar granule cells. Journal of Neuroscience 20:7208-7219. PMC6772767   PubMed
  7. Creed, S., and M. McKenzie. 2019. Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM). In Cancer Metabolism: Methods and Protocols. M. Haznadar, editor. Springer New York, New York, NY. 69-76. PubMed
  8. Perry, S. W., J. P. Norman, J. Barbieri, E. B. Brown, and H. A. Gelbard. 2011. Mitochondrial membrane potential probes and the proton gradient: a practical usage guide. BioTechniques 50:98-115.  PMC3115691   PubMed
  9. Chazotte, B. 2011. Labeling Mitochondria with TMRM or TMRE. Cold Spring Harbor Protocols 2011:pdb.prot5641     PubMed
  10. Crowley, L. C., M. E. Christensen, and N. J. Waterhouse. 2016. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harbor Protocols 2016:pdb.prot087361   PubMed