Structure and Spectra of Di-4-ANEPPDHQ

  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 472 nm, 615 nm.
  • Absorbance, emission peaks in ethanol: 519 nm, 733 nm.
  • Recommended excitation range: 510-530 nm (click here for more on how to choose the wavelength).
  • MW: 666.55 g/mol

Unique Packaging for Convenience and Reliability

  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (67 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant
Packaging of Di-4-ANEPPS, Potentiometric Probes

Order information

  • See table for pricing
  • Contact sales@potentiometricprobes.com for a quote
  • Minimum order: 10. Price break: 100 tubes
  • Shipping not included
  • Other quantities and formats available upon request
  • Inquire about “Academic Discount” for academic researchers
Di-4-ANEPPDHQ, 100 nmol Pre-dried Aliquot Pricing
Order Quantity (tubes) Price / Tube Order Total ($US)
10 $25.00 $250
100 $21.00 $2100
Di-4-ANEPPDHQ, Bulk Pricing
Order Quantity (mg) Price / mg Order Total ($US)
1 $225 $225
5 $180 $900


1. Fisher, J. A. N., J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras, and B. M. Salzberg. 2008. Two-Photon Excitation of Potentiometric Probes Enables Optical Recording of Action Potentials From Mammalian Nerve Terminals In Situ. J Neurophysiol 99:1545-1553. PubMed

2. Jin, L., A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew. 2006. Characterization and application of a new optical probe for membrane lipid domains. Biophysical journal 90:2563-2575. PubMed

3. Jin, L., A. C. Millard, J. P. Wuskell, H. A. Clark, and L. M. Loew. 2005. Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics. Biophysical journal 89:L04-06. PubMed

4. Obaid, A. L., L. M. Loew, J. P. Wuskell, and B. M. Salzberg. 2004. Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis. J Neurosci Methods 134:179-190. PubMed

5. Kwiatek, J. M., D. M. Owen, A. Abu-Siniyeh, P. Yan, L. M. Loew, and K. Gaus. 2013. Characterization of a new series of fluorescent probes for imaging membrane order. PLoS ONE 8:e52960.3563652. PubMed

6. Owen, D. M., P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee. 2006. Fluorescence Lifetime Imaging Provides Enhanced Contrast when Imaging the Phase-Sensitive Dye di-4-ANEPPDHQ in Model Membranes and Live Cells. Biophys. J. 90:L80-82. PubMed


Potentiometric Probes develops and supplies voltage-sensitive dyes (VSDs) for academic and pharmaceutical industry researchers. These fluorescent dyes are used to stain cells, tissues, and whole organs and allow electrical activity of the brain and heart to be studied optically using microscopes. Voltage-sensitive dyes are used in basic research, as well as for drug discovery and cardiac safety screening of developmental drugs.

Potentiometric Probes is developing new VSD technology that promises to have rapid response as well as high sensitivity for the highest resolution and most informative optical voltage recordings possible.