Highlighted Application

 

Data from Dr. Corey Acker (1), showing a mouse pyramidal neuron loaded with ElectroFluor-530sTM, and an optical voltage recording from a single dendritic spine. This more soluble version of ElectroFluor-530 allows loading of individual neurons through the patch pipette, making it possible to do voltage recordings from spines and axons.

 

Structure and Spectra of ElectroFluor-530sTM

Chemical structure of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
Spectra in MLVs of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 441 nm, 611 nm.
  • Recommended excitation wavelengths: 530 nm, 1060 nm (2P) (click here for more on how to choose the wavelength).
  • MW: 623.48 g/mol
  • Alternative names: Di-2-AN(F)EPTEA, PY3243

Unique Packaging for Convenience and Reliability

 

 

  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (62 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant

Order information

  • See table for pricing
  • Contact sales@potentiometricprobes.com for a quote
  • Minimum order: 10. Price break: 100 tubes
  • Shipping not included
  • Other quantities and formats available upon request
  • Inquire about “Academic Discount” for academic researchers
ElectroFluor-530s/Di-2-AN(F)EPPTEA – 100 nmol Pre-dried Aliquot Pricing
Product Number Order Quantity (tubes) Price / Tube Order Total ($US)
32430 10 $20.00 $200
32430 100 $17.00 $1700
ElectroFluor-530s/Di-2-AN(F)EPPTEA, Bulk Pricing
Product Number Order Quantity (mg) Price / mg Order Total ($US)
32435 1 $200 $200
32436 5 $160 $800

Citations

  1. Acker, C. D., E. Hoyos, and L. M. Loew. 2016. EPSPs Measured in Proximal Dendritic Spines of Cortical Pyramidal Neurons. eNeuro 3(2). Pubmed
  2. Rowan, M. J., E. Tranquil, and J. M. Christie. 2014. Distinct kv channel subtypes contribute to differences in spike signaling properties in the axon initial segment and presynaptic boutons of cerebellar interneurons. J Neurosci. Pubmed
  3. Yan, P., C. D. Acker, W. L. Zhou, P. Lee, C. Bollensdorff, A. Negrean, J. Lotti, L. Sacconi, S. D. Antic, P. Kohl, H. D. Mansvelder, F. S. Pavone, and L. M. Loew. 2012. Palette of fluorinated voltage-sensitive hemicyanine dyes. Proceedings of the National Academy of Sciences of the United States of America. Pubmed