Structure and Spectra of Di-4-ANBDQBS

Chemical structure of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
Spectra in MLVs of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 531 nm, 722 nm.
  • Absorbance, emission peaks in ethanol: 572 nm, no fluorescence.
  • Recommended excitation range: 620-660 nm (click here for more on how to choose the wavelength).
  • MW: 570.78 g/mol
  • Other names: JPW-6033

Unique Packaging for Convenience and Reliability



  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (57 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant

Order information


1. Lopez-Izquierdo, A., M. Warren, M. Riedel, S. Cho, S. Lai, R. L. Lux, K. W. Spitzer, I. J. Benjamin, M. Tristani-Firouzi, and C. J. Jou. 2014. A near-infrared fluorescent voltage-sensitive dye allows for moderate-throughput electrophysiological analyses of human-induced pluripotent stem cell-derived cardiomyocytes. Am J Physiol Heart Circ Physiol. PubMed
2. Matiukas, A., B. G. Mitrea, M. Qin, A. M. Pertsov, A. G. Shvedko, M. D. Warren, A. V. Zaitsev, J. P. Wuskell, M. D. Wei, J. Watras, and L. M. Loew. 2007. Near-infrared voltage-sensitive fluorescent dyes optimized for optical mapping in blood-perfused myocardium. Heart Rhythm. PubMed
3. Warren, M., K. W. Spitzer, B. W. Steadman, T. D. Rees, P. Venable, T. Taylor, J. Shibayama, P. Yan, J. P. Wuskell, L. M. Loew, and A. V. Zaitsev. 2010. High-precision recording of the action potential in isolated cardiomyocytes using the near-infrared fluorescent dye di-4-ANBDQBS. Am J Physiol Heart Circ Physiol. PubMed
4. Aras, K. K., N. R. Faye, B. Cathey, and I. R. Efimov. 2018. Critical Volume of Human Myocardium Necessary to Maintain Ventricular Fibrillation. Circ Arrhythm Electrophysiol. PubMed
5. Lee, P., J. G. Quintanilla, J. M. Alfonso-Almazán, C. Galán-Arriola, P. Yan, J. Sánchez-González, N. Pérez-Castellano, J. Pérez-Villacastín, B. Ibañez, L. M. Loew, and D. Filgueiras-Rama. 2019. In-Vivo Ratiometric Optical Mapping Enables High-Resolution Cardiac Electrophysiology in Pig Models. Cardiovasc Res. PubMed