Structure and Spectra of Di-4-ANEPPS

Chemical structure of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
Spectra in MLVs of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 470 nm, 620 nm.
  • Absorbance, emission peaks in ethanol: 500 nm, 725 nm.
  • Recommended excitation range: 510-530 nm (click here for more on how to choose the wavelength).
  • MW: 480.66 g/mol

Unique Packaging for Convenience and Reliability



  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (48 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant

Order information

  • See table for pricing
  • Contact for a quote
  • Minimum order: 20. Price break: 100 tubes
  • Shipping not included
  • Other quantities and formats available upon request
  • Inquire about “Academic Discount” for academic researchers
Di-4-ANEPPS – 100 nmol Pre-dried Aliquot Pricing
Product Number Order Quantity (tubes) Price / Tube Order Total ($US)
02110 20 $5.00 $100
02110 100 $4.50 $450
Di-4-ANEPPS, Bulk Pricing
Product Number Order Quantity (mg) Price / mg Order Total ($US)
02115 1 $80 $80
02116 5 $60 $300


1. Hortigon-Vinagre, M. P., V. Zamora, F. L. Burton, J. Green, G. A. Gintant, and G. L. Smith. 2016. The Use of Ratiometric Fluorescence Measurements of the Voltage Sensitive Dye Di-4-ANEPPS to Examine Action Potential Characteristics and Drug Effects on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Toxicol Sci. Pubmed
2. Blinova, K., J. Stohlman, J. Vicente, D. Chan, L. Johannesen, M. P. Hortigon-Vinagre, V. Zamora, G. Smith, W. J. Crumb, L. Pang, B. Lyn-Cook, J. Ross, M. Brock, S. Chvatal, D. Millard, L. Galeotti, N. Stockbridge, and D. G. Strauss. 2017. Comprehensive Translational Assessment of Human-Induced Pluripotent Stem Cell Derived Cardiomyocytes for Evaluating Drug-Induced Arrhythmias. Toxicol Sci. Pubmed
3. Bachtel, A. D., R. A. Gray, J. M. Stohlman, E. B. Bourgeois, A. E. Pollard, and J. M. Rogers. 2011. A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di-4-ANEPPS using pulsed LED excitation. IEEE Trans Biomed Eng. Pubmed
4. Fluhler, E., V. G. Burnham, and L. M. Loew. 1985. Spectra, membrane binding, and potentiometric responses of new charge shift probes. Biochemistry. Pubmed
5. Montana, V., D. L. Farkas, and L. M. Loew. 1989. Dual-wavelength ratiometric fluorescence measurements of membrane potential. Biochemistry. Pubmed
6. Loew, L. M., L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu. 1992. A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J Membr Biol. Pubmed