Structure and Spectra of Di-4-ANEPPS

Structure of Di-4-ANEPPS, Potentiometric Probes
Spectra of Di-4-ANEPPS, Potentiometric Probes
  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 470 nm, 620 nm.
  • Absorbance, emission peaks in ethanol: 500 nm, 725 nm.
  • Recommended excitation range: 510-530 nm (click here for more on how to choose the wavelength).
  • MW: 480.66 g/mol

Unique Packaging for Convenience and Reliability

  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (48 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant
Packaging of Di-4-ANEPPS, Potentiometric Probes

Order information

  • See table for pricing
  • Contact sales@potentiometricprobes.com for a quote
  • Minimum order: 20. Price break: 100 tubes
  • Shipping not included
  • Other quantities and formats available upon request
  • Inquire about “Academic Discount” for academic researchers
Di-4-ANEPPS, 100 nmol Pre-dried Aliquot Pricing
Order Quantity (tubes) Price / Tube Order Total ($US)
20 $5.00 $100
100 $4.50 $450
Di-4-ANEPPS, Bulk Pricing
Order Quantity (mg) Price / mg Order Total ($US)
1 $80 $80
5 $60 $300


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2. Blinova, K., J. Stohlman, J. Vicente, D. Chan, L. Johannesen, M. P. Hortigon-Vinagre, V. Zamora, G. Smith, W. J. Crumb, L. Pang, B. Lyn-Cook, J. Ross, M. Brock, S. Chvatal, D. Millard, L. Galeotti, N. Stockbridge, and D. G. Strauss. 2017. Comprehensive Translational Assessment of Human-Induced Pluripotent Stem Cell Derived Cardiomyocytes for Evaluating Drug-Induced Arrhythmias. Toxicol Sci 155(1):234-247. Pubmed
3. Bachtel, A. D., R. A. Gray, J. M. Stohlman, E. B. Bourgeois, A. E. Pollard, and J. M. Rogers. 2011. A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di-4-ANEPPS using pulsed LED excitation. IEEE Trans Biomed Eng 58(7):2120-2126. Pubmed
4. Fluhler, E., V. G. Burnham, and L. M. Loew. 1985. Spectra, membrane binding, and potentiometric responses of new charge shift probes. Biochemistry 24(21):5749-5755. Pubmed
5. Montana, V., D. L. Farkas, and L. M. Loew. 1989. Dual-wavelength ratiometric fluorescence measurements of membrane potential. Biochemistry 28(11):4536-4539. Pubmed
6. Loew, L. M., L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu. 1992. A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J Membr Biol 130(1):1-10. Pubmed