Highlighted Application

From Crocini et al. 2014 (2) Figure 3: Delay of Ca 2+ release in AP-failing TT of acute detubulated cells.

(A) TPF image of a stained rat ventricular myocyte after formamide-induced osmotic shock; membrane potential optical recording in magenta and [Ca2+]i in green. (Scale bar, 5 μm.)

(B) Average of 10 subsequent fluorescence traces (ΔF/F0) of voltage (magenta) and [Ca2+]i (green) recorded at the three different sites indicated in A: SS and two TTi. APs are elicited at 200 ms (black arrowheads).

(C) Superposition of the Ca2+ traces nearby SS and TT1 (Top) and of SS and TT2 (Middle). (Bottom) A close-up of TT2 and SS superposition (interval indicated by dashed line in the middle trace).

Structure and Spectra of ElectroFluor530TM

Chemical structure of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
Spectra in MLVs of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 447 nm, 616 nm.
  • Absorbance, emission peaks in ethanol: 481 nm, 725 nm.
  • Recommended excitation wavelengths: 530 nm, 1060 nm (2P) (click here for more on how to choose the wavelength).
  • MW: 679.59 g/mol

Unique Packaging for Convenience and Reliability



  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (68 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant

Order information

  • See table for pricing
  • Contact sales@potentiometricprobes.com for a quote
  • Minimum order: 10. Price break: 100 tubes
  • Shipping not included
  • Other quantities and formats available upon request
  • Inquire about “Academic Discount” for academic researchers
ElectroFluor530/Di-4-AN(F)EPPTEA – 100 nmol Pre-dried Aliquot Pricing – #31740
Product Number Order Quantity (tubes) Price/ Tube Order Total ($US)
31740 10 $20.00 $200
31740 100 $17.00 $1700
ElectroFluor530/Di-4-AN(F)EPPTEA, Bulk Pricing
Product Number Order Quantity (mg) Price / mg Order Total ($US)
31745 1 $200 $200
31746 5 $160 $800


  1. Yan, P., C. D. Acker, W. L. Zhou, P. Lee, C. Bollensdorff, A. Negrean, J. Lotti, L. Sacconi, S. D. Antic, P. Kohl, H. D. Mansvelder, F. S. Pavone, and L. M. Loew. 2012. Palette of fluorinated voltage-sensitive hemicyanine dyes. Proceedings of the National Academy of Sciences of the United States of America. Pubmed
  2. Crocini, C., R. Coppini, C. Ferrantini, P. Yan, L. M. Loew, C. Tesi, E. Cerbai, C. Poggesi, F. S. Pavone, and L. Sacconi. 2014. Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure. Proc Natl Acad Sci U S A. Pubmed