Structure and Spectra of TMRM

Chemical structure of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
Spectra in MLVs of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 559 nm, 589 nm.
  • Absorbance, emission peaks in ethanol: 551 nm, 579 nm.
  • MW: 500.9 g/mol

Unique Packaging for Convenience and Reliability



  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (50 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant
  • Bulk mg quantities also available

Order information

  • See table for pricing
  • Contact for a quote
  • Minimum order: 20. Price break: 100 tubes
  • Shipping not included
  • Other quantities and formats available upon request
  • Inquire about “Academic Discount” for academic researchers
TMRM – 100 nmol Pre-dried Aliquot Pricing
Product Number Order Quantity (tubes) Price / Tube Order Total ($US)
01230 20 $4.00 $80
01230 100 $3.50 $350
TMRM, Bulk Pricing
Product Number Order Quantity (mg) Price / mg Order Total ($US)
01235 1 $50 $50
01236 5 $40 $200



  1. ​Ehrenberg, B., V. Montana, M. D. Wei, J. P. Wuskell, and L. M. Loew. 1988. Membrane potential can be determined in individual cells from the nernstian distribution of cationic dyes. Biophysical journal. PubMed
  2. Farkas, D. L., M. D. Wei, P. Febbroriello, J. H. Carson, and L. M. Loew. 1989. Simultaneous imaging of cell and mitochondrial membrane potentials. Biophysical journal. PubMed
  3. Loew, L. M., R. A. Tuft, W. Carrington, and F. S. Fay. 1993. Imaging in five dimensions: time-dependent membrane potentials in individual mitochondria. Biophysical journal PubMed
  4. Loew, L. M., W. Carrington, R. A. Tuft, and F. S. Fay. 1994. Physiological cytosolic Ca2+ transients evoke concurrent mitochondrial depolarizations. Proceedings of the National Academy of Sciences of the United States of America 
  5. Fink, C., F. Morgan, and L. M. Loew. 1998. Intracellular fluorescent probe concentrations by confocal microscopy. Biophysical journal. PubMed
  6. Ward, M. W., A. C. Rego, B. G. Frenguelli, and D. G. Nicholls. 2000. Mitochondrial membrane potential and glutamate excitotoxicity in cultured cerebellar granule cells. Journal of Neuroscience 20:7208-7219. PubMed
  7. Creed, S., and M. McKenzie. 2019. Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM). In Cancer Metabolism: Methods and Protocols. M. Haznadar, editor. Springer New York, New York, NY. PubMed
  8. Perry, S. W., J. P. Norman, J. Barbieri, E. B. Brown, and H. A. Gelbard. 2011. Mitochondrial membrane potential probes and the proton gradient: a practical usage guide. BioTechniques. PubMed
  9. Chazotte, B. 2011. Labeling Mitochondria with TMRM or TMRE. Cold Spring Harbor Protocols. PubMed
  10. Crowley, L. C., M. E. Christensen, and N. J. Waterhouse. 2016. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harbor Protocols. PubMed