Structure and Spectra of Di-4-ANEPPDHQ

Chemical structure of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
Spectra in MLVs of voltage-sensitive dye Di-4-AN(F)EP(F)PTEA from Potentiometric Probes
  • Absorbance, emission peaks in multilamellar lipid vesicles (MLVs) shown above: 472 nm, 615 nm.
  • Absorbance, emission peaks in ethanol: 519 nm, 733 nm.
  • Recommended excitation range: 510-530 nm (click here for more on how to choose the wavelength).
  • MW: 666.55 g/mol

Unique Packaging for Convenience and Reliability



  • Pre-dried aliquots (solid, solvent removed)
  • Quantity: 100 nmol / tube (67 μg)
  • 2.0-mL free standing polypropylene tubes
    • Sealed cap
    • Individually labeled
    • Bagged with desiccant

Order information



  • See table for pricing
  • Contact for a quote
  • Minimum order: 10. Price break: 100 tubes
  • Shipping not included
  • Other quantities and formats available upon request
  • Inquire about “Academic Discount” for academic researchers
Di-4-ANEPPDHQ – 100 nmol Pre-dried Aliquot Pricing 
Product Number Order Quantity (tubes) Price / Tube Order Total ($US)
50290 20 $7.50 $150
50290 100 $6.50 $650
Di-4-ANEPPDHQ, Bulk Pricing
Product Number Order Quantity (mg) Price / mg Order Total ($US)
50295 1 $100 $100
50296 5 $80 $400


 1. Fisher, J. A. N., J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras, and B. M. Salzberg. 2008. Two-Photon Excitation of Potentiometric Probes Enables Optical Recording of Action Potentials From Mammalian Nerve Terminals In Situ. J Neurophysiol. PubMed

2. Jin, L., A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew. 2006. Characterization and application of a new optical probe for membrane lipid domains. Biophysical journal. PubMed

3. Jin, L., A. C. Millard, J. P. Wuskell, H. A. Clark, and L. M. Loew. 2005. Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics. Biophysical journal. PubMed

4. Obaid, A. L., L. M. Loew, J. P. Wuskell, and B. M. Salzberg. 2004. Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis. J Neurosci Methods. PubMed

5. Kwiatek, J. M., D. M. Owen, A. Abu-Siniyeh, P. Yan, L. M. Loew, and K. Gaus. 2013. Characterization of a new series of fluorescent probes for imaging membrane order. PLoS ONE. PubMed

6. Owen, D. M., P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee. 2006. Fluorescence Lifetime Imaging Provides Enhanced Contrast when Imaging the Phase-Sensitive Dye di-4-ANEPPDHQ in Model Membranes and Live Cells. Biophys. J. PubMed